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Título:
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Development and analysis of furazolidone-resistant Escherichia coli mutants
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Autores:
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Martinez-Puchol, Sandra ;
Gomes, Cláudia ;
Pons, Maria J. ;
Ruiz-Roldan, Lidia ;
Torrents de la Pena, Alba ;
Ochoa, Theresa J. ;
Ruiz, Joaquim
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Tipo de documento:
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texto impreso
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Editorial:
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Wiley, 2019-02-06T14:52:12Z
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Nota general:
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info:eu-repo/semantics/restrictedAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
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Idiomas:
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Inglés
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Palabras clave:
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Editados por otras instituciones
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Artículos
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Artículos en revistas indizadas
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Resumen:
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Furazolidone-resistant mutants were obtained from four clinical isolates of diarrhoeagenic Escherichia coli. The stability of the resistance and the frequency of mutation were established. The minimal inhibitory concentration of furazolidone, nitrofurantoin, nalidixic acid, ampicillin, chloramphenicol and tetracycline was established both in the presence and absence of the efflux pump inhibitor Phe-Arg-beta-Naphtylamyde. The presence of mutations in the nitroreductase genes nfsA and nfsB was analysed by PCR; sequencing and their enzymatic activity was assessed by a spectrophotometric assay. Alterations in outer membrane proteins were studied by SDS-PAGE. The frequency of mutation ranged from
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En línea:
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http://doi.org/10.1111/apm.12401
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