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Título:
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Molecular specific and sensitive detection of pyrazinamide and its metabolite pyrazinoic acid by means of surface enhanced raman spectroscopy employing in situ prepared colloids
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Autores:
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Muehlig, A. ;
Jahn, I.J. ;
Heidler, J. ;
Jahn, M. ;
Weber, K. ;
Sheen, P. ;
Zimic, M. ;
Cialla-May, D. ;
Popp, J.
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Tipo de documento:
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texto impreso
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Editorial:
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MDPI, 2019-08-08T15:23:44Z
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Nota general:
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info:eu-repo/semantics/restrictedAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
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Idiomas:
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Inglés
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Palabras clave:
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Editados por otras instituciones
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Artículos
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Artículos en revistas indizadas
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Resumen:
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The prodrug pyrazinamide (PZA) is metabolized by the mycobacteria to pyrazinoic acid (POA), which is expelled into the extracellular environment. PZA resistance is highly associated to a lack of POA efflux. Thus, by detecting a reduction of the concentration of POA in the extracellular environment, by means of lab-on-a-chip (LoC)-SERS (surface-enhanced Raman spectroscopy), an alternative approach for the discrimination of PZA resistant mycobacteria is introduced. A droplet-based microfluidic SERS device has been employed to illustrate the potential of the LoC-SERS method for the discrimination of PZA resistant mycobacteria. The two analytes were detected discretely in aqueous solution with a limit of detection of 27 ?m for PZA and 21 ?m for POA. The simultaneous detection of PZA and POA in aqueous mixtures could be realized within a concentration range from 20 ?m to 50 ?m for PZA and from 50 ?m to 80 ?m for POA. © 2019 by the authors.
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En línea:
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http://doi.org/10.3390/app9122511
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