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Título:
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Second-line drug susceptibility breakpoints for Mycobacterium tuberculosis using the MODS assay
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Autores:
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Trollip, A. P. ;
Moore, D. ;
Coronel, J. ;
Caviedes, L. ;
Klages, S. ;
Victor, T. ;
Romancenco, E. ;
Crudu, V. ;
Ajbani, K. ;
Vineet, V. P. ;
Rodrigues, C. ;
Jackson, R. L. ;
Eisenach, K. ;
Garfein, R. S. ;
Rodwell, T. C. ;
Desmond, E. ;
Groessl, E. J. ;
Ganiats, T. G. ;
Catanzaro, A.
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Tipo de documento:
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texto impreso
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Editorial:
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International Union Against Tuberculosis and Lung Disease, 2020-06-10T18:11:35Z
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Nota general:
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info:eu-repo/semantics/restrictedAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
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Idiomas:
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Inglés
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Palabras clave:
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Editados por otras instituciones
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Artículos
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Artículos en revistas indizadas
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Resumen:
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OBJECTIVE: To establish breakpoint concentrations for the fluoroquinolones (moxifloxacin [MFX] and ofloxacin [OFX]) and injectable second-line drugs (amikacin [AMK], kanamycin [KM] and capreomycin [CPM]) using the microscopic observation drug susceptibility (MODS) assay. SETTING: A multinational study conducted between February 2011 and August 2012 in Peru, India, Moldova and South Africa. DESIGN: In the first phase, breakpoints for the fluoroquinolones and injectable second-line drugs (n = 58) were determined. In the second phase, MODS second-line drug susceptibility testing (DST) as an indirect test was compared to MGIT DST (n = 89). In the third (n = 30) and fourth (n = 156) phases, we determined the reproducibility and concordance of MODS second-line DST directly from sputum. RESULTS: Breakpoints for MFX (0.5 mug/ml), OFX (1 mug/ml), AMK (2 mug/ml), KM (5 mug/ml) and CPM (2.5 mug/ml) were determined. In all phases, MODS results were highly concordant with MGIT DST. The few discrepancies suggest that the MODS breakpoint concentrations for some drugs may be too low. CONCLUSION: MODS second-line DST yielded comparable results to MGIT second-line DST, and is thus a promising alternative. Further studies are needed to confirm the accuracy of the drug breakpoints and the reliability of MODS second-line DST as a direct test.
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En línea:
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http://doi.org/10.5588/ijtld.13.0229
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