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Título:
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C57BL/6 ?-1,3-Galactosyltransferase knockout mouse as an animal model for experimental Chagas disease
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Autores:
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Valencia Ayala, Edward ;
Rodrigues da Cunha, Gisele ;
Araujo Azevedo, Maira ;
Calderón, Maritza ;
Jimenez, Juan ;
Venuto, Ana Paula ;
Gazzinelli, Ricardo ;
Ynocente Lavalle, Raúl Jesus ;
Vidal Riva, Angela Giovana ;
Hincapie, Robert ;
Finn, M. G. ;
Marques, Alexandre F.
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Tipo de documento:
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texto impreso
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Editorial:
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American Chemical Society, 2020-07-14T00:02:36Z
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Nota general:
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info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
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Idiomas:
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Inglés
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Palabras clave:
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Editados por otras instituciones
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Artículos
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Artículos en revistas indizadas
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Resumen:
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The leading animal model of experimental Chagas disease, the mouse, plays a significant role in studies for vaccine development, diagnosis, and human therapies. Humans, along with Old World primates, alone among mammals, cannot make the terminal carbohydrate linkage of the ?-Gal trisaccharide. It has been established that the anti-?-Gal immune response is likely to be a critical factor for protection against Trypanosoma cruzi (T. cruzi) infection in humans. However, the mice customarily employed for the study of T. cruzi infection naturally express the ?-Gal epitope and therefore do not produce anti-?-Gal antibodies. Here, we used the C57BL/6 ?-1,3-galactosyltransferase knockout (?-GalT-KO) mouse, which does not express the ?-Gal epitope as a model for experimental Chagas disease. We found the anti-?-Gal IgG antibody response to an increase in ?-GalT-KO mice infected with Arequipa and Colombiana strains of T. cruzi, leading to fewer parasite nests, lower parasitemia, and an increase of INF-?, TNF-?, and IL-12 cytokines in the heart of ?-GalT-KO mice compared with ?-GalT-WT mice on days 60 and 120 postinfection. We therefore agree that the C57BL/6 ?-GalT-KO mouse represents a useful model for initial testing of therapeutic and immunological approaches against different strains of T. cruzi.
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En línea:
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http://repositorio.upch.edu.pe/handle/upch/8355
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