|
Título:
|
Trypomastigote Excretory Secretory Antigen (TESA) blot is associated in neonates with parasite load and detects congenital T. cruzi infection using anti-SAPA IgM
|
|
Autores:
|
Noazin, Sassan ;
Lee, Jessica A. ;
Malaga, Edith S. ;
Ayala, Edward Valencia ;
Condori, Beth J. ;
Roca, Cristian ;
Lescano, Andres G. ;
Bern, Caryn ;
Castillo, Walter ;
Mayta, Holger ;
Menduiña, Maria Carmen ;
Verastegui, Manuela R. ;
Tinajeros, Freddy ;
Gilman, Robert H. ;
Chagas Working Group in Bolivia and Peru
|
|
Tipo de documento:
|
texto impreso
|
|
Editorial:
|
Oxford University Press, 2018-12-03T17:02:50Z
|
|
Nota general:
|
info:eu-repo/semantics/restrictedAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
|
|
Idiomas:
|
Inglés
|
|
Palabras clave:
|
Editados por otras instituciones
,
Artículos
,
Artículos en revistas indizadas
|
|
Resumen:
|
Background: Congenital Trypanosoma cruzi accounts for an estimated 22% of new cases in Latin America. However, neonatal diagnosis is challenging, as 9-month follow-up for IgG testing is poor, qPCR is not routinely performed, and micromethod misses more than 40% of congenital infections. Methods: Biorepository samples from new mothers and their infants from Piura, Peru (non-endemic area), and Santa Cruz, Bolivia (endemic) were accessed. Infant specimens were assessed using micromethod, qPCR and TESA blot for IgM SAPA bands, using qPCR as the gold standard. Results: When compared to qPCR, IgM TESA blot was both sensitive and specific for congenital Chagas diagnosis. Cumulative sensitivity (whether only four bands or all six bands were present) was 80% (59-92%). Specificity was 94% (92-96%) in endemic areas and 100% in non-endemic. SAPA bands occurred sequentially, and in pairs, and parasite load correlated highly with the number of SAPA bands present. Micromethod detected less than half of infected infants. Conclusions: The IgM blot for SAPA bands demonstrates that SAPA antigen may be able to provide a Point of Care test for congenital infection that is rapid, relatively inexpensive, and more sensitive than micromethod.
|
|
En línea:
|
http://doi.org/10.1093/infdis/jiy562
|
