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Título:
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Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity
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Autores:
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Mota-Martorell, Natalia ;
Jové, Mariona ;
Pradas, Irene ;
Sánchez, Isabel ;
Gómez, José ;
Naudi, Alba ;
Barja de Quiroga, Gustavo ;
Pamplona, Reinald
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Tipo de documento:
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texto impreso
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Editorial:
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Elsevier, 2020-04-20
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Dimensiones:
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application/pdf
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Nota general:
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cc_by_nc_nd
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Biología: Bioquímica
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Materia = Ciencias Biomédicas: Biología: Fisiología animal
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Materia = Ciencias Biomédicas: Biología: Mamíferos
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Tipo = Artículo
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Resumen:
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Mitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.
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En línea:
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https://eprints.ucm.es/id/eprint/61317/1/Mota-Martorell%2C%20Natalia%20et%20al.%202020.%20Low%20abundance%20of%20NDUFV2.....pdf
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