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Autor Reche, Pedro A |
Documentos disponibles escritos por este autor (43)
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ADP-ribosyltransferases (ADPRTs) form an interesting class of enzymes with well-established roles as potent bacterial toxins and metabolic regulators. ADPRTs catalyze the transfer of the ADP-ribose moiety from NAD(+) onto specific substrates inc[...]![]()
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Yang, Hailin ; Kim, Sung-Kwon ; Kim, Mikyung ; Reche, Pedro A ; Morehead, Tiara J ; Damon, Inger K ; Welsh, Raymond M ; Reinherz, Ellis L | American Society for Clinical Investigation | 2005The EGF-like domain of smallpox growth factor (SPGF) targets human ErbB-1, inducing tyrosine phosphorylation of certain host cellular substrates via activation of the receptor's kinase domain and thereby facilitating viral replication. Given the[...]![]()
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Kim, Mikyung ; Yang, Hailin ; Kim, Sung-Kwon ; Reche, Pedro A ; Tirabassi, Rebecca S ; Hussey, Rebecca E ; Chishti, Yasmin ; Rheinwald, James G ; Morehead, Tiara J ; Zech, Tobias ; Damon, Inger K ; Welsh, Raymond M ; Reinherz, Ellis L | American Society for Biochemistry and Molecular Biology | 2004Variola, the causative agent of smallpox, is a highly infectious double-stranded DNA virus of the orthopox genus that replicates within the cytoplasm of infected cells. For unknown reasons prominent skin manifestations, including "pox," mark the[...]![]()
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Reche, Pedro A ; Arrebola, R ; Olmo, A ; Santi, D V ; González-Pacanowska, D. ; Ruiz Pérez, Luis Miguel | Elsevier | 1994We have cloned, sequenced and expressed the Trypanosoma cruzi gene encoding the bifunctional protein dihydrofolate reductase-thymidylate synthase (DHFR-TS). The strategy followed for the isolation of positive clones from a genomic library was ba[...]![]()
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Proteasomes play a central role in the major histocompatibility class I (MHCI) antigen processing pathway. They conduct the proteolytic degradation of proteins in the cytosol, generating the C-terminus of CD8 T cell epitopes and MHCI-peptide lig[...]![]()
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Identification of peptides that can bind to major histocompatibility complex (MHC) molecules is important for anticipation of T-cell epitopes and for the design of epitope-based vaccines. Population coverage of epitope vaccines is, however, comp[...]![]()
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Moody, A M ; Chui, D ; Reche, Pedro A ; Priatel, J J ; Marth, J D ; Reinherz, Ellis L | 2001The functional consequences of glycan structural changes associated with cellular differentiation are ill defined. Herein, we investigate the role of glycan adducts to the O-glycosylated polypeptide stalk tethering the CD8alphabeta coreceptor to[...]![]()
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Reche, Pedro A ; Keskin, Derin B ; Hussey, Rebecca E ; Ancuta, Petronela ; Gabuzda, Dana ; Reinherz, Ellis L | BioMed Central | 2006Cytotoxic T lymphocytes (CTL) protect against viruses including HIV-1. To avoid viral escape mutants that thwart immunity, we chose 25 CTL epitopes defined in the context of natural infection with functional and/or structural constraints that ma[...]![]()
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We introduced previously an on-line resource, RANKPEP that uses position specific scoring matrices (PSSMs) or profiles for the prediction of peptide-MHC class I (MHCI) binding as a basis for CD8 T-cell epitope identification. Here, using PSSMs t[...]![]()
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Reche, Pedro A ; Zhang, Hong ; Glutting, John-Paul ; Reinherz, Ellis L | Oxford University Press | 2005EPIMHC is a relational database of MHC-binding peptides and T cell epitopes that are observed in real proteins. Currently, the database contains 4867 distinct peptide sequences from various sources, including 84 tumor-associated antigens. The EP[...]![]()
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Reche, Pedro A ; Arrebola, R ; Santi, D V ; González-Pacanowska, D. ; Ruiz Pérez, Luis Miguel | Elsevier | 1995We have cloned and expressed in Escherichia coli a 702-base pair gene coding for the dihydrofolate reductase (DHFR) domain of the bifunctional dihydrofolate reductase-thymidylate synthase (DHFR-TS) from Trypanosoma cruzi. The DHFR domain was pur[...]![]()
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Zhong, Weimin ; Reche, Pedro A ; Lai, Char-Chang ; Reinhold, Bruce ; Reinherz, Ellis L | American Society for Biochemistry and Molecular Biology | 2003A genome-wide search using major histocompatibility complex (MHC) class I binding and proteosome cleavage site algorithms identified 101 influenza A PR8 virus-derived peptides as potential epitopes for CD8+ T cell recognition in the H-2b mouse. [...]![]()
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The lipoyl domains of 2-oxo acid dehydrogenase multienzyme complexes and the biotinyl domains of biotin-dependent enzymes have homologous structures, but the target lysine residue in each domain is correctly selected for posttranslational modifi[...]![]()
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Soumelis, Vassili ; Reche, Pedro A ; Kanzler, Holger ; Yuan, Wei ; Edward, Gina ; Homey, Bernhart ; Gilliet, Michel ; Ho, Steve ; Antonenko, Svetlana ; Lauerma, Annti ; Smith, Kathleen ; Gorman, Daniel ; Zurawski, Sandra M. ; Abrams, Jon ; Menon, Satish ; McClanahan, Terri ; Waal-Malefyt, Rene de ; Bazan, Fernando ; Kastelein, Robert A. ; Liu, Yong-Jun | Nature Publishing Group | 2002Whether epithelial cells play a role in triggering the immune cascade leading to T helper 2 (T(H)2)-type allergic inflammation is not known. We show here that human thymic stromal lymphopoietin (TSLP) potently activated CD11c(+) dendritic cells [...]![]()
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Reche, Pedro A ; Soumelis, Vassili ; Gorman, D M ; Clifford, T ; Liu, MR ; Travis, M ; Zurawski, Sandra M. ; Johnston, J ; Liu, Yong-Jun ; Spits, H ; Waal-Malefyt, Rene de ; Kastelein, Robert A. ; Bazan, Fernando | The American Association of Immunologists | 2001The sequence of a novel hemopoietic cytokine was discovered in a computational screen of genomic databases, and its homology to mouse thymic stromal lymphopoietin (TSLP) suggests that it is the human orthologue. Human TSLP is proposed to signal [...]![]()
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Otto, Helge ; Reche, Pedro A ; Bazan, Fernando ; Dittmar, Katharina ; Haag, Friedrich ; Koch-Nolte, Friedrich | 2005BACKGROUND: ADP-ribosylation is an enzyme-catalyzed posttranslational protein modification in which mono(ADP-ribosyl)transferases (mARTs) and poly(ADP-ribosyl)transferases (pARTs) transfer the ADP-ribose moiety from NAD onto specific amino acid [...]![]()
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Immunoinformatics is an emerging new field that benefits from computational analyses and tools that facilitate the understanding of the immune system. A large number of immunoinformatics resources such as immune-related databases and analysis so[...]![]()
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Diez Rivero, Carmen ; García Boronat, María ; Reche, Pedro A | Biomedical Informatics Publishing Group | 2008Immunoinformatics is an emerging new field that benefits from computational analyses and tools that facilitate the understanding of the immune system. A large number of immunoinformatics resources such as immune-related databases and analysis so[...]![]()
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Arrebola, R ; Olmo, A ; Reche, Pedro A ; Garvey, Edward P. ; Santi, D V ; Ruiz Pérez, Luis Miguel ; González-Pacanowska, D. | American Society for Biochemistry and Molecular Biology | 1994-04-08The MTX-resistant Leishmania major promastigote cell line D7BR1000 displays extrachromosomal amplified R-region DNA, which contains the gene for dihydrofolate reductase-thymidylate synthase (DHFR-TS) (Garvey, E. P., and Santi, D. V. (1986) Scien[...]![]()
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Arrebola, R ; Olmo, A ; Reche, Pedro A ; Garvey, Edward P. ; Santi, D V ; Ruiz Pérez, Luis Miguel ; González-Pacanowska, D. | 1994The MTX-resistant Leishmania major promastigote cell line D7BR1000 displays extrachromosomal amplified R-region DNA, which contains the gene for dihydrofolate reductase-thymidylate synthase (DHFR-TS) (Garvey, E. P., and Santi, D. V. (1986) Scien[...]![]()
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Reche, Pedro A | 2000Biotin and lipoic acid moieties are the covalently attached coenzyme cofactors of several multicomponent enzyme complexes that catalyze key metabolic reactions. Attachment of these moieties to the biotinyl- and lipoyl-dependent enzymes is post-t[...]![]()
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Fiorini, Emma ; Reche, Pedro A | 2002Negative selection eliminates thymocytes bearing autoreactive T cell receptors (TCR) via an apoptotic mechanism. We have cloned an inhibitor of NF-kappa B, I kappa BNS, which is rapidly expressed upon TCR-triggered but not dexamethasone- or gamm[...]![]()
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Fridkis-Hareli, Masha ; Reche, Pedro A ; Reinherz, Ellis L | 2004During development, thymocytes carrying TCRs mediating low-affinity interactions with MHC-bound self-peptides are positively selected for export into the mature peripheral T lymphocyte pool. Thus, exogenous administration of certain altered pept[...]![]()
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Reche, Pedro A ; Reinherz, Ellis L | 2005Prediction of peptide binding to major histocompatibility complex (MHC) molecules is a basis for anticipating T-cell epitopes, as well as epitope discovery-driven vaccine development. In the human, MHC molecules are known as human leukocyte anti[...]![]()
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DNA methylation plays a key role in the regulation of gene expression. The most common type of DNA modification consists of the methylation of cytosine in the CpG dinucleotide. At the present time, there is no method available for the prediction[...]![]()
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Peptides that bind to a given major histocompatibility complex (MHC) molecule share sequence similarity. Therefore, a position specific scoring matrix (PSSM) or profile derived from a set of peptides known to bind to a specific MHC molecule woul[...]![]()
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T cell immune responses are driven by the recognition of peptide antigens (T cell epitopes) that are bound to major histocompatibility complex (MHC) molecules. T cell epitope immunogenicity is thus contingent on several events, including appropr[...]![]()
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Reche, Pedro A ; Reinherz, Ellis L | 2007Prediction of peptide binding to major histocompatibility complex (MHC) molecules is a basis for anticipating T-cell epitopes. Peptides that bind to a given MHC molecule are related by sequence similarity. Therefore, a position-specific scoring [...]![]()
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García Boronat, María ; Díez Rivero, Carmen M. ; Reinherz, Ellis L ; Reche, Pedro A | Oxford University Press | 2008We have developed PVS (Protein Variability Server), a web-based tool that uses several variability metrics to compute the absolute site variability in multiple protein-sequence alignments (MSAs). The variability is then assigned to a user-select[...]![]()
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The transport of peptides to the endoplasmic reticulum by the transporter associated with antigen processing (TAP) is a necessary step towards determining CD8 T cell epitopes. In this work, we have studied the predictive performance of support v[...]![]()
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Bhasin, Manoj ; Reinherz, Ellis L ; Reche, Pedro A | 2006Histones are DNA-binding proteins found in the chromatin of all eukaryotic cells. They are highly conserved and can be grouped into five major classes: H1/H5, H2A, H2B, H3, and H4. Two copies of H2A, H2B, H3, and H4 bind to about 160 base pairs [...]![]()
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Martínez Naves, Eduardo ; Lafuente Duarte, Esther ; Reche, Pedro A | Federation of European Biochemical Societies | 2011Functional characterization of proteins belonging to the MHC I superfamily involves knowing their cognate ligands, which can be peptides, lipids or none. However, the experimental identification of these ligands is not an easy task and generally[...]![]()
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Reductive acetylation of the lipoyl domain (E2plip) of the dihydrolipoyl acetyltransferase component of the pyruvate dehydrogenase multienzyme complex of Escherichia coli is catalysed specifically by its partner pyruvate decarboxylase (E1p), and[...]![]()
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In this report,we present 15 patients with histological and immunopathologically proven pemphigus vulgaris (PV). After a mean of 80 months since the onset of disease, when evaluated serologically, they had antibodies typical of PV and pemphigoid[...]![]()
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Biotin-dependent enzymes contain a biotinyl-lysine residue in a conserved sequence motif, MKM, located in a surface hairpin turn in one of the two beta-sheets that make up the domain. A sub-gene encoding the 82-residue C-terminal biotinyl domain[...]![]()
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Major histocompatibility complex class I (MHCI) and class II (MHCII) molecules display peptides on antigen-presenting cell surfaces for subsequent T-cell recognition. Within the human population, allelic variation among the classical MHCI and II[...]![]()
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The lipoyl domains of the dihydrolipoyl acyltransferase (E2p, E2o) components of the pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes are specifically recognised by their cognate 2-oxo acid decarboxylase (E1p, E1o). A prominent su[...]![]()
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The post-translational attachment of biotin and lipoic acid to specific lysine residues displayed in protruding beta-turns in homologous biotinyl and lipoyl domains of their parent enzymes is catalysed by two different ligases. We have expressed[...]![]()
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Glowacki, Gustavo ; Braren, Rickmer ; Firner, Kathrin ; Nissen, Marion ; Kühl, Maren ; Reche, Pedro A ; Bazan, Fernando ; Cetkovic-Cvrlje, Marina ; Leiter, Edward ; Haag, Friedrich ; Koch-Nolte, Friedrich | 2002ADP-ribosyltransferases including toxins secreted by Vibrio cholera, Pseudomonas aerurginosa, and other pathogenic bacteria inactivate the function of human target proteins by attaching ADP-ribose onto a critical amino acid residue. Cross-specie[...]![]()
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García Fuentes, Luis ; Reche, Pedro A ; López Mayorga, O ; Santi, D V ; González-Pacanowska, D. ; Barón, C | Wiley InterScience | 1995The binding of 5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP) to Lactobacillus casei recombinant thymidylate synthase has been studied by isothermal titration microcalorimetry at pH 7.1 over the temperature range 16-35 degrees C. Calorimetric[...]![]()
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Sheikh, Qamar M ; Gatherer, Derek ; Reche, Pedro A ; Flower, Darren R | Oxford University Pres | 2016-07Motivation: Influenza A viral heterogeneity remains a significant threat due to unpredictable antigenic drift in seasonal influenza and antigenic shifts caused by the emergence of novel subtypes. Annual review of multivalent influenza vaccines t[...]