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Biophysical and biological impact on the structure and IgE-binding of the interaction of the olive pollen allergen Ole e 7 with lipids

Oeo-Santos, Carmen ; López Rodríguez, Juan C. ; García-Mouton, Cristina ; San Segundo-Acosta, Pablo ; Jurado, Aurora ; Moreno-Aguilar, Carmen ; García-Álvarez, Begoña ; Pérez Gil, Jesús ; Villalba, Mayte ; Barderas Manchado, Rodrigo ; Cruz Rodríguez, Antonio | Elsevier | 2020-03-04

Ole e 7 allergen from Olea europaea pollen possesses a major clinical relevance because it produces severe symptoms, such as anaphylaxis, in allergic patients exposed to high olive pollen counts. Ole e 7 is a non-specific lipid transfer protein [...]

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Protein and lipid fingerprinting of native-like membrane complexes by combining thin layer chromatography and protein electrophoresis: the example of lung surfactant

López-Rodríguez, Elena ; Roldán López, Nuria ; García-Álvarez, Begoña ; Pérez-Gil, Jesús | American Society for Biochemistry and Molecular Biology | 2019-02

Traditionally, thin layer chromatography (TLC) has been used for the analysis of lipids isolated from membrane complexes. Here, we describe a method based on the combination of TLC and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) for the qu[...]

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