Información del autor
Documentos disponibles escritos por este autor (5)
Añadir el resultado a su cesta Hacer una sugerencia Refinar búsqueda
texto impreso
Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples sim[...]texto impreso
Guevara, Govinda ; Fernández de las Heras, Laura ; Perera González, Julián ; Navarro LLorens, Juana María | Elsevier | 2017-09The 3-Ketosteroid-9?-Hydroxylase, also known as KshAB [androsta-1,4-diene-3,17-dione, NADH:oxygen oxidoreductase (9?-hydroxylating); EC 1.14.13.142)], is a key enzyme in the general scheme of the bacterial steroid catabolism in combination with [...]texto impreso
Guevara, Govinda ; Fernández de las Heras, Laura ; Perera González, Jesús ; Navarro LLorens, Juana María | BioMed Central | 2017-03-14Background: The Rhodococcus ruber strain Chol-4 genome contains at least three putative 3-ketosteroid ?1 - dehydrogenase ORFs (kstD1, kstD2 and kstD3) that code for flavoenzymes involved in the steroid ring degradation. The aim of this work is t[...]texto impreso
Guevara, Govinda ; Olortegui Flores, Yamileth ; Fernández de las Heras, Laura ; Perera González, Julián ; Navarro LLorens, Juana María | Public Library of Science | 2019-07-26Rhodococcus ruber Chol-4 is a potent steroid degrader that has a great potential as a biotechnological tool. As proof of concept, this work presents testosterone production from 4- androstene-3,17-dione by tailoring innate catabolic enzymes of t[...]texto impreso
Guevara, Govinda ; Castillo López, María ; Alonso, Sergio ; Perera González, Julián ; Navarro LLorens, Juana María | BMC | 2019Background: Rhodococcus ruber strain Chol-4, a strain isolated from a sewage sludge sample, is able to grow in minimal medium supplemented with several compounds, showing a broad catabolic capacity. We have previously determined its genome seque[...]
