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Wrent, Petra ; Rivas, Eva María ; Gil de Prado, Elena ; Martínez Peinado, José ; Siloniz Jiménez, María Isabel de | MDPI | 2015-09In this work we analyze the spoiling potential of Meyerozyma guilliermondii in yogurt. The analysis was based on contaminated samples sent to us by an industrial laboratory over two years. All the plain and fruit yogurt packages were heavily con[...]texto impreso
Development of an affordable typing method for Meyerozymaguilliermondii using microsatellite markers
Wrent, Petra ; Rivas, Eva María ; Martínez Peinado, José ; Siloniz Jiménez, María Isabel de | Elsevier | 2016-01-09Despite previously published methods, there is still a lack of rapid and affordable methods for genotyping the Meyerozyma guilliermondii yeast species. The development of microsatellite markers is a useful genotyping method in several yeast spec[...]texto impreso
Wrent, Petra ; Rivas, Eva María ; Gil de Prado, Elena ; Martínez Peinado, José ; Siloniz Jiménez, María Isabel de | Elsevier | 2015-01-16In this work, we developed a specific PCR assay for Debaryomyces hansenii strains that uses a putative homologous PAD1 region (729 bp) present in this yeast species as a target. The amplification of this sequence with the D. hansenii specific pr[...]texto impreso
Rodrigues, B. ; Lima-Costa, M. E. ; Constantino, A. ; Raposo, S. ; Felizardo, C. ; Gonçalves, D. ; Fernandes, T. ; Dionísio, L. ; Martínez Peinado, José | Elsevier | 2016-10Alcoholic fermentation of carob waste sugars (sucrose, glucose and fructose) extracted with cheese whey, by co-cultures of Saccharomyces cerevisiae and Kluyveromyces lactis has been analyzed. Growth and fermentation of S. cerevisiae in the carob[...]texto impreso
Rodrigues, B. ; Martínez Peinado, José ; Raposo, S. ; Constantino, A. ; Quintas, C. ; Lima Costa, M. E. | Korean Society for Microbiology and Biotechnology | 2015Carob waste is a useful raw material for the second-generation ethanol because 50% of its dry weight is sucrose, glucose, and fructose. To optimize the process, we have studied the influence of the initial concentration of sugars on the fermenta[...]texto impreso
The specific consumption rate of substrate, as well as the associated specific growth rate, is an essential parameter in the mathematical description of substrate-limited microbial growth. In this paper we develop a completely new kinetic model [...]texto impreso
Gil de Prado, Elena ; Rivas, Eva María ; Siloniz Jiménez, María Isabel de ; Diezma, Belén ; Barreiro, Pilar ; Martínez Peinado, José | John Wiley & Sons | 2014-11The colony shape of four yeast species growing on agar medium was measured for 116 days by image analysis. Initially, all the colonies are circular, with regular edges. The loss of circularity can be quantitatively estimated by the eccentricity [...]texto impreso
Rivas, Eva María ; Gil de Prado, Elena ; Wrent, Petra ; Siloniz Jiménez, María Isabel de ; Correa, E.C. ; Conejero Meca, Francisco ; Murciano Cespedosa, Antonio ; Martínez Peinado, José | John Wiley & Sons | 2014-12We propose a model, based on the Gompertz equation, to describe the growth of yeasts colonies on agar medium. This model presents several advantages: (i) one equation describes the colony growth, which previously needed two separate ones (linear[...]texto impreso
Wrent, Petra ; Rivas, Eva María ; Martínez Peinado, José ; Siloniz Jiménez, María Isabel de | Elsevier | 2010-08-15Unlike previously reported methods that need a combination of several typing techniques, we have 22 developed a single method for strain typing of the Zygosaccharomyces bailii, Z. mellis and Z. rouxii spoilage 23 species. Strains belonging to ot[...]texto impreso
Wrent, Petra ; Rivas, Eva María ; Martínez Peinado, José ; Siloniz Jiménez, María Isabel de | Elsevier | 2017-01-16Based on IGS-PCR RFLP polymorphism, we previously detected two Z. rouxiistrains (CECT 11923 and CECT 10425) that clustered with hybrid strains (NCYC 1682, NCYC 3060 and NCYC 3061). Given the recently recognized important industrial role of hybri[...]
