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Título:
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Development of an immobilized biocatalyst based on Bacilluspsychrosaccharolyticus NDT for the preparative synthesis oftrifluridine and decytabine
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Autores:
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Fresco-Taboada, Alba ;
Serra, Immacolata ;
Arroyo Sánchez, Miguel ;
Fernández-Lucas, Jesús ;
Mata Riesco, Isabel de la ;
Terreni, Marco
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Tipo de documento:
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texto impreso
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Editorial:
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Elsevier, 2016-01
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/restrictedAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Biología: Bioquímica
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Tipo = Artículo
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Resumen:
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The immobilization of Bacillus psychrosaccharolyticus nucleoside 2 -deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600 Da agarose followed by crosslinking with 70% oxidized dextran (20 kDa). The percentage of recovered activity was further improved (from 21% to 33%) by the addition of 20% glycerol to the immobilization mixture. The resulting biocatalyst showed a stability profile similar to that of the soluble enzyme and it was used for the preparative synthesis oftrifluridine and decytabine obtaining conversions ranging from 50% to 76%.
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En línea:
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https://eprints.ucm.es/id/eprint/43694/1/Fresco-Taboada%2C%20A.%20Development%20of%20an%20immobilized.pdf
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