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Fresco-Taboada, Alba ; Serra, Immacolata ; Arroyo Sánchez, Miguel ; Fernández-Lucas, Jesús ; Mata Riesco, Isabel de la ; Terreni, Marco | Elsevier | 2016-01The immobilization of Bacillus psychrosaccharolyticus nucleoside 2 -deoxyribosyltransferase was deeply investigated and finally optimized. The best immobilization procedure resulted to be ionic adsorption on PEI 600 Da agarose followed by crossl[...]texto impreso
Rinaldi, Francesca ; Fernández-Lucas, Jesús ; Fuente, Diego de la ; Zheng, Changping ; Bavaro, Teodora ; Peters, Benjamin ; Massolini, Gabriella ; Annunziata, Francesca ; Conti, Paola ; Mata, Isabel de la ; Terreni, Marco ; Calleri, Enrica | Elsevier | 2020-03-26In this work, a mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) were developed as prototypes for biosynthetic purposes and their performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest wer[...]texto impreso
Fresco Taboada, Alba ; Serra, Immacolata ; Fernández Lucas, Jesús ; Acebal Sarabia, Carmen ; Arroyo, Miguel ; Terreni, Marco ; Mata Riesco, Isabel de la | MDPI | 2014-07-31Nucleoside 2'-deoxyribosyltransferase (NDT) from the psychrophilic bacterium Bacillus psychrosaccharolyticus CECT 4074 has been cloned and produced for the first time. A preliminary characterization of the recombinant protein indicates that the [...]
