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Título:
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Electrochemical immunosensor for sensitive determination oftransforming growth factor (TGF) - ?1 in urine
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Autores:
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Sánchez Tirado, Esther ;
Martínez García, Gonzalo ;
González Cortés, Araceli ;
Yáñez Sedeño, Paloma ;
Pingarrón Carrazón, José Manuel
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Tipo de documento:
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texto impreso
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Editorial:
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Elsevier, 2017-02
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/restrictedAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias: Química
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Materia = Ciencias: Química: Química analítica
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Tipo = Artículo
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Resumen:
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The first amperometric immunosensor for the quantification of TGF-?1, a cytokine proposed as a biomarker for patients having or at risk for renal disease, is described in this work. The immunosensor design involves disposable devices using carboxylic acid-functionalized magnetic microparticles supported onto screen-printed carbon electrodes and covalent immobilization of the specific antibody for TGF-?1 using Mix&Go polymer. A sandwich-type immunoassay was performed using biotin-anti-TGF and conjugation with peroxidase-labeled streptavidin (poly-HRP-Strept) polymer. Amperometric measurements were carried out at 0.20 V by adding hydrogen peroxide solution onto the electrode surface in the presence of hydroquinone as the redox mediator. The calibration plot allowed a range of linearity extending between 15 and 3000 pg/mL TGF-?1 which is adequate for the determination of the cytokine in plasma and urine. The limit of detection, 10 pg/mL, is notably improved with respect to those obtained with ELISA kits. The usefulness of the immunosensor for the determination of low TGF-?1 concentrations in real samples was evaluated by analyzing spiked urine at different pg/mL concentration levels.
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En línea:
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https://eprints.ucm.es/41229/1/BB%202016%20TGF%20%20MBs.pdf
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