|
Título:
|
Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway.
|
|
Autores:
|
Sanz, A. Belén ;
García, Raúl ;
Rodríguez-Peña, José Manuel ;
Diez-Muñiz, Sonia ;
Nombela, César ;
Peterson, Craig L. ;
Arroyo, Javier
|
|
Tipo de documento:
|
texto impreso
|
|
Fecha de publicación:
|
2012-05-23
|
|
Dimensiones:
|
application/pdf
|
|
Nota general:
|
cc_by_nc_sa
info:eu-repo/semantics/openAccess
|
|
Idiomas:
|
|
|
Palabras clave:
|
Estado = Publicado
,
Materia = Ciencias Biomédicas: Farmacia: Microbiología
,
Tipo = Artículo
|
|
Resumen:
|
In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating the transcriptional response regulated by Rlm1. swi/snf mutants show drastically reduced expression of cell wall stress-responsive genes and hypersensitivity to cell wall-interfering compounds. On stress, binding of RNA Pol II to the promoters of these genes depends on Rlm1, Slt2, and SWI/SNF. Rlm1 physically interacts with SWI/SNF to direct its association to target promoters. Finally, we observe nucleosome displacement at the CWI-responsive gene MLP1/KDX1, which relies on the SWI/SNF complex. Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress.
|
|
En línea:
|
https://eprints.ucm.es/id/eprint/21895/1/Sanz-MBC.pdf
|
