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Título:
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“Super p53” Mice Display Retinal Astroglial Changes
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Autores:
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Salazar Corral, Juan José ;
Gallego-Pinazo, Roberto ;
Hoz Montañana, María Rosa de ;
Pinazo-Durán, María Dolores ;
Rojas López, María Blanca ;
Ramírez Sebastián, Ana Isabel ;
Serrano Espinosa, Manuel ;
Ramirez Sebastian, Jose Manuel
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Tipo de documento:
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texto impreso
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Editorial:
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Public Library Science, 2013-06-07
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Dimensiones:
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application/pdf
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Nota general:
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cc_by
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Medicina: Genética médica
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Materia = Ciencias Biomédicas: Medicina: Neurociencias
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Materia = Ciencias Biomédicas: Medicina: Oftalmología
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Materia = Ciencias Biomédicas: Óptica y optometría: Anatomía ocular
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Tipo = Artículo
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Resumen:
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Tumour-suppressor genes, such as the p53 gene, produce proteins that inhibit cell division under adverse conditions, as in the case of DNA damage, radiation, hypoxia, or oxidative stress (OS). The p53 gene can arrest proliferation and trigger death by apoptosis subsequent to several factors. In astrocytes, p53 promotes cell-cycle arrest and is involved in oxidative stress-mediated astrocyte cell death. Increasingly, astrocytic p53 is proving fundamental in orchestrating neurodegenerative disease pathogenesis. In terms of ocular disease, p53 may play a role in hypoxia due to ischaemia and may be involved in the retinal response to oxidative stress (OS). We studied the influence of the p53 gene in the structural and quantitative characteristics of astrocytes in the retina. Adult mice of the C57BL/6 strain (12 months old) were distributed into two groups: 1) mice with two extra copies of p53 (“super p53”; n = 6) and 2) wild-type p53 age-matched control, as the control group (WT; n = 6). Retinas from each group were immunohistochemically processed to locate the glial fibrillary acidic protein (GFAP). GFAP+ astrocytes were manually counted and the mean area occupied for one astrocyte was quantified. Retinal-astrocyte distribution followed established patterns; however, morphological changes were seen through the retinas in relation to p53 availability. The mean GFAP+ area occupied by one astrocyte in “super p53” eyes was significantly higher (p
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En línea:
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https://eprints.ucm.es/id/eprint/31161/1/Plos%20One_super%20p53.pdf
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