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Título:
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Screening and Identification of Potential Sex-AssociatedSequences in Danio rerio
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Autores:
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Luzio, Ana ;
Coimbra, Ana M. ;
Benito Jiménez, Cesar ;
Fontaínhas Fernandes, Antonio A. ;
Matos, Manuela
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Tipo de documento:
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texto impreso
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Editorial:
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Wiley Online Library, 2015-06-16
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/restrictedAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Biología: Genética
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Materia = Ciencias Biomédicas: Biología: Peces
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Tipo = Artículo
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Resumen:
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Current knowledge on zebrafish (Danio rerio) suggests that sex determination has a polygenic genetic basis in this species, although environmental factors may also be involved. This study aimed to identify sex-associated genomic regions using two different marker systems: inter-simple sequence repeats (ISSRs) and random-amplified polymorphic DNA (RAPDs). Two bulks were constructed: one with DNA from zebrafish females and the other from males; then, a total of 100 ISSR and 280 RAPD primers were tested. Three DNA fragments presenting sexual dimorphism (female-linked: OPA17436 and OPQ191027 ; male-linked: OPQ19951 ) were determined from sequential analysis of the bulks followed by assessment in individuals. These fragments were cloned and convert into the following sequenced characterized amplified regions (SCAR): DrSM_F1, DrSM_F2, and DrSM_M, which share identities with sequences located in chromosomes 2, 3, and 11 (Zv9), respectively. Using these potential markers in zebrafish samples it was possible to correctly identify 80% of the males (DrSM_M) and 100% of the females (DrSM_F1?+?DrSM_F2) in the analyzed population.
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En línea:
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https://eprints.ucm.es/42867/1/Lucio.%202015.%20Screening%20and%20identification%20of%20potential%20sex-associated%20sequences%20in%20Danio%20rerio.pdf
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