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Reche, Pedro A ; Arrebola, R ; Olmo, A ; Santi, D V ; González-Pacanowska, D. ; Ruiz Pérez, Luis Miguel | Elsevier | 1994We have cloned, sequenced and expressed the Trypanosoma cruzi gene encoding the bifunctional protein dihydrofolate reductase-thymidylate synthase (DHFR-TS). The strategy followed for the isolation of positive clones from a genomic library was ba[...]texto impreso
Arrebola, R ; Olmo, A ; Reche, Pedro A ; Garvey, Edward P. ; Santi, D V ; Ruiz Pérez, Luis Miguel ; González-Pacanowska, D. | American Society for Biochemistry and Molecular Biology | 1994-04-08The MTX-resistant Leishmania major promastigote cell line D7BR1000 displays extrachromosomal amplified R-region DNA, which contains the gene for dihydrofolate reductase-thymidylate synthase (DHFR-TS) (Garvey, E. P., and Santi, D. V. (1986) Scien[...]texto impreso
Arrebola, R ; Olmo, A ; Reche, Pedro A ; Garvey, Edward P. ; Santi, D V ; Ruiz Pérez, Luis Miguel ; González-Pacanowska, D. | 1994The MTX-resistant Leishmania major promastigote cell line D7BR1000 displays extrachromosomal amplified R-region DNA, which contains the gene for dihydrofolate reductase-thymidylate synthase (DHFR-TS) (Garvey, E. P., and Santi, D. V. (1986) Scien[...]
