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Título:
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Rod-Like Microglia Are Restricted to Eyes with Laser-Induced Ocular Hypertension but Absent from the Microglial Changes in the Contralateral Untreated Eye
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Autores:
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Hoz Montañana, María Rosa de ;
Gallego Collado, Beatriz Isabel ;
Ramírez Sebastián, Ana Isabel ;
Rojas López, María Blanca ;
Salazar Corral, Juan José ;
Valiente Soriano, Francisco Javier ;
Avilés Trigueros, Marcelino ;
Villegas Pérez, María Paz ;
Vidal Sanz, Manuel ;
Triviño Casado, Alberto ;
Ramirez Sebastian, Jose Manuel
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Tipo de documento:
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texto impreso
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Editorial:
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Public Library Science, 2013-12-18
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Dimensiones:
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application/pdf
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Nota general:
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cc_by
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Medicina: Genética médica
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Materia = Ciencias Biomédicas: Medicina: Neurociencias
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Materia = Ciencias Biomédicas: Medicina: Oftalmología
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Materia = Ciencias Biomédicas: Óptica y optometría: Anatomía ocular
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Tipo = Artículo
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Resumen:
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In the mouse model of unilateral laser-induced ocular hypertension (OHT) the microglia in both the treated and the normotensive untreated contralateral eye have morphological signs of activation and up-regulation of MHC-II expression in comparison with naive. In the brain, rod-like microglia align to less-injured neurons in an effort to limit damage. We investigate whether: i) microglial activation is secondary to laser injury or to a higher IOP and; ii) the presence of rod-like microglia is related to OHT. Three groups of mice were used: age-matched control (naive, n=15); and two lasered: limbal (OHT, n=15); and non-draining portion of the sclera (scleral, n=3). In the lasered animals, treated eyes as well as contralateral eyes were analysed. Retinal whole-mounts were immunostained with antibodies against, Iba-1, NF-200, MHC-II, CD86, CD68 and Ym1. In the scleral group (normal ocular pressure) no microglial signs of activation were found. Similarly to naive eyes, OHT-eyes and their contralateral eyes had ramified microglia in the nerve-fibre layer related to the blood vessel. However, only eyes with OHT had rod-like microglia that aligned end-to-end, coupling to form trains of multiple cells running parallel to axons in the retinal surface. Rod-like microglia were CD68+ and were related to retinal ganglion cells (RGCs) showing signs of degeneration (NF-200+ RGCs). Although MHC-II expression was up-regulated in the microglia of the NFL both in OHT-eyes and their contralateral eyes, no expression of CD86 and Ym1 was detected in ramified or in rod-like microglia. After 15 days of unilateral lasering of the limbal and the non-draining portion of the sclera, activated microglia was restricted to OHT-eyes and their contralateral eyes. However, rod-like microglia were restricted to eyes with OHT and degenerated NF-200+ RGCs and were absent from their contralateral eyes. Thus, rod-like microglia seem be related to the neurodegeneration associated with HTO.
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En línea:
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https://eprints.ucm.es/id/eprint/31191/1/Plos%20One_Rod%20like.pdf
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