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Título:
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Solid lipid nanoparticles for ocular delivery of isoniazid: evaluation, proof of concept and in vivo safety & kinetics
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Autores:
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Singh, Mandeep ;
Guzmán Aránguez, Ana Isabel ;
Hussain, Afzal ;
Srinivas, Cheerneni S. ;
Kaur, Indu P.
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Tipo de documento:
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texto impreso
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Editorial:
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Future Medicine, 2019-01-29
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias: Química: Bioquímica
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Materia = Ciencias Biomédicas: Óptica y optometría: Anatomía ocular
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Tipo = Artículo
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Resumen:
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Aim: Evaluation of solid lipid nanoparticles (SLNs) for ocular delivery of isoniazid (INH). Materials & methods: INH-SLNs were characterized for morphological, thermal, crystalline and nuclear magnetic resonance properties. In vitro release and ex vivo corneal permeability of INH-SLNs was also evaluated. Proof-of-concept uptake studies were performed in corneal and conjunctival cell lines and in vivo in rat eye using fluorescein-labeled SLNs. Antimycobacterial activity of INH-SLNs was confirmed. In vivo aqueous humor pharmacokinetics, toxicity and tolerance was performed in rabbit/rat eye. Results: INH-SLNs showed extended release (48 h), enhanced corneal permeability (1.6-times), five-times lower MIC, significant in vitro and in vivo uptake of fluorescein-labeled SLNs, 4.2-times ocular bioavailability (area under the curve) and in vivo acute and repeat dose safety. Conclusion: INH-SLNs are an effective ocular delivery system.
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En línea:
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https://eprints.ucm.es/55110/1/Ana%20Isabel%20Guzman%20Aranguez-preprint-solid%20lipid2019.pdf
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