| Título: | Determination of B-N-oxalyl-L-a, B-diaminopropionic acid and homoarginine in Lathyrus sativus and Lathyrus cicera by capillary zone electrophoresis |
| Autores: | Sacristán San Cristóbal, Mara ; Varela, Alejandro ; Martín Pedrosa, Mercedes ; Burbano, Carmen ; Cuadrado Vives, María del Carmen ; Legaz González, María Estrella ; Muzquiz, Mercedes |
| Tipo de documento: | texto impreso |
| Editorial: | Wiley, 2015-05 |
| Dimensiones: | application/pdf |
| Nota general: | info:eu-repo/semantics/restrictedAccess |
| Idiomas: | |
| Palabras clave: | Estado = Publicado , Materia = Ciencias Biomédicas: Biología: Botánica , Materia = Ciencias Biomédicas: Biología: Fisiología vegetal , Tipo = Artículo |
| Resumen: |
BACKGROUND: Lathyrus species as legumes represent an alternative protein source for human and animal nutrition. Heavy consumption of these species can lead to lathyrism, caused by the non-protein amino acid B-N-oxalyl-L-a, B-diaminopropionic acid (B-ODAP). Currently, there is no well-defined level below which B-ODAP is considered non-toxic. In this work, the B-ODAP content was determined in L. sativus and L. cicera samples to assess their potential toxicity. Homoarginine is another non-protein amino acid found in Lathyrus spp. with interesting implications for human and animal nutrition. RESULTS: The level of B-ODAP found in these two species ranged from 0.79 to 5.05 mg g?1. The homoarginine content of the samples ranged from 7.49 to 12.44 mg g?1. CONCLUSION: This paper describes an accurate, fast and sensitive method of simultaneous detection and quantification of B-ODAP and homoarginine by capillary zone electrophoresis in L. cicera and L. sativus seeds. Moreover, several methods of extraction were compared to determine the highest performance. |
| En línea: | https://eprints.ucm.es/47140/1/Sacrist%C3%A1n%2C%20%20et%20al.%202014.%20Determination%20of%20B-N-oxalyl.pdf |
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