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Título:
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Tetrahymena Glutathione peroxidase family: a comparative analysis of these antioxidant enzymes and differential gene expression to metalsand Oxidizing Agents
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Autores:
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Cubas-Gaona, Liliana L. ;
Francisco Martínez, Patricia de ;
Martín-González, Ana María ;
Gutiérrez Fernández, Juan Carlos
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Tipo de documento:
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texto impreso
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Editorial:
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MDPI, 2020-07-05
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Dimensiones:
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application/pdf
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Nota general:
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cc_by
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Biología: Genética
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Materia = Ciencias Biomédicas: Biología: Invertebrados
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Materia = Ciencias Biomédicas: Biología: Microbiología
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Tipo = Artículo
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Resumen:
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In the present work, an extensive analysis of the putative glutathione peroxidases (GPx) of the eukaryotic microorganism model Tetrahymena thermophila is carried out. A comparative analysis with GPx present in other Tetrahymena species and other very taxonomically diverse ciliates is also performed. A majority of ciliate GPx have replaced the selenocysteine (Sec) by Cys in its catalytic center, so they can be considered as phospholipid hydroperoxide glutathione peroxidases (PHGPx). Selenocysteine insertion sequence (SECIS) elements have been detected in several ciliate GPx that do not incorporate Sec in their amino acid sequences, and conversely, in other ciliate GPx with Sec, no SECIS elements are detected. These anomalies are analyzed and discussed. From the phylogenetic analysis using the ciliate GPx amino acid sequences, the existence of extensive intraand interspecific gene duplications that produced multiple GPx isoforms in each species is inferred. The ancestral character of the selenoproteins is also corroborated. The analysis by qRT-PCR of six selected T. thermophila GPx genes has shown a quantitative differential expression between them, depending on the stressor (oxidizing agents, apoptotic inducer or metals) and the time of exposure.
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En línea:
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https://eprints.ucm.es/id/eprint/62539/1/Cubas-Gaona%2C%20Liliana%20M.%20et%20al.%202020.%20Tetrahymena%20Glutathione%20Peroxidase%20family.....pdf
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