| Título: | Corneal Re-epithelialization Stimulated by Diadenosine Polyphosphates Recruits RhoA/ROCK and ERK1/2 Pathways |
| Autores: | Mediero Muñoz, Aránzazu ; Guzmán Aránguez, Ana Isabel ; Crooke, Almudena ; Peral Cerdá, Assumpta ; Pintor, Jesús |
| Tipo de documento: | texto impreso |
| Editorial: | The Association for Research in Vision and Ophthalmology, Inc., 2008-11 |
| Dimensiones: | application/pdf |
| Nota general: |
cc_by_nc_nd info:eu-repo/semantics/openAccess |
| Idiomas: | |
| Palabras clave: | Estado = Publicado , Materia = Ciencias: Química , Materia = Ciencias: Química: Biología molecular , Materia = Ciencias Biomédicas: Medicina: Oftalmología , Tipo = Artículo |
| Resumen: |
Purpose. To investigate the role of ERK1/2 and RhoA/ROCK intracellular pathways in the modification of corneal re-epithelialization when stimulated by the diadenosine polyphosphates Ap4A and Ap3A. Methods. In wounded confluent SIRC (Statens Seruminstitut rabbit cornea) cell monolayers and in the presence or absence of Ap4A or Ap3A 100 ?M, a battery of P2 receptor antagonists and inhibitors of tyrosin kinases, MAPK, and cytoskeleton pathways (AG1478 100 ?M, U0126 100 ?M, Y27632 100 nM, and (?)-blebbistatin 10 ?M; n = 8 each) were assayed. Also, the activation of ERK1/2 and ROCK-I was examined by Western blot assay after treatment with Ap4A and Ap3A (100 ?M), with or without suramin, RB-2, U0126, and Y27632. The intracellular distribution of pERK and ROCK-I was examined in the presence of Ap4A or Ap3A (100 ?M) with U0126 and Y27632 (100 nM). Results. In the presence of Ap4A, U0126, Y27632, AG1478, and (?)-blebbistatin, reduced the migration rate compared to the effect of Ap4A alone (P Conclusions. The activation of the Ap4A/P2Y2 receptor, accelerates corneal epithelial cell migration during wound healing with the activation of MAPK and cytoskeleton pathways, whereas activation of the Ap3A/P2Y6 receptor signals only the MAPK pathway. |
| En línea: | https://eprints.ucm.es/39507/1/Corneal%20reepitheliaization_IOVS-2011.pdf |
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