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Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Ortega, Sara ; Pacheco, Beatriz ; Peterson, Darrell L. ; Gavilanes, Francisco | Academic Press Inc. Elsevier Science | 2010-06Hepatitis C virus encodes two enveloped glycoproteins, E1 and E2, which are involved in viral attachment and entry into target cells. We have obtained in insect cells infected by recombinant baculovirus a chimeric secreted recombinant protein, E[...]texto impreso
Olombrada Sacristán, Miriam ; Herrero Galán, Elías ; Tello, Daniel ; Oñaderra Sánchez, Mercedes ; Gavilanes, José G. ; Martínez del Pozo, Álvaro ; García Ortega, Lucía | Elsevier | 2013Fungal ribotoxins were discovered almost 50 years ago as extracellular ribonucleases (RNases) with antitumoral properties. However, the biological function of these toxic proteins has remained elusive. The discovery of the ribotoxin HtA, produce[...]texto impreso
Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Ortega, Sara ; Lombana, Laura ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Peterson, Darrell L. ; Gavilanes, Francisco | Wiley-Blackwell | 2014-06-01We have used an isolated chimeric protein E1340E2661 that includes the ectodomains of the envelope proteins of epatitis C virus to study its interaction with model membranes. E1340E2661 has some of the membrane destabilization properties, vesicl[...]texto impreso
Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2015-03-01In this report it is described for the first time the expression and purification of large quantities of a oluble and correctly folded chimeric recombinant protein, E2661E1340, containing the permuted Hepatitis C virus (HCV) glycoprotein ectodom[...]texto impreso
Hernansanz Agustín, Pablo ; Choya Foces, Carmen ; Carregal Romero, Susana ; Ramos, Elena ; Oliva, Tamara ; Villa Piña, Tamara ; Moreno, Laura ; Izquierdo Alvarez, Alicia ; Cabrera Garcia, J.Daniel ; Cortés, Ana ; Lechuga Vieco, Ana Victoria ; Jadiya, Pooja ; Navarro, Elisa ; Parada, Esther ; Palomino Antolín, Alejandra ; Tello, Daniel ; Acín Pérez, Rebeca ; Rodríguez Aguilera, Juan Carlos ; Navas, Plácido ; Cogolludo, Angel ; López Montero, Iván ; Martínez del Pozo, Álvaro ; Egea, Javier ; López, Manuela G. ; Elrod, John W. ; Ruiz Cabello, J. ; Bogdanova, Anna ; Enríquez, José Antonio ; Martínez Ruiz, Antonio | Nature Research | 2020-07-29All metazoans depend on O2 delivery and consumption by the mitochondrial oxidative phosphorylation (OXPHOS) system to produce energy. A decrease in O2 availability (hypoxia) leads to profound metabolic rewiring. In addition, OXPHOS uses O2 to pr[...]texto impreso
Rodríguez-Rodríguez, Mar ; Tello, Daniel ; Yélamos, Belén ; GómezGutiérrez, Julián ; Pacheco, Beatriz ; Ortega, Sara ; Serrano, Alicia G. ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2009-01We describe the structural and antigenic properties of a soluble form of hepatitis C virus E2 envelope protein ectodomain ending at residue 661 (E2661) which is obtained in large quantities in a baculovirus/insect cell system. The protein is sec[...]
