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Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Ortega, Sara ; Pacheco, Beatriz ; Peterson, Darrell L. ; Gavilanes, Francisco | Academic Press Inc. Elsevier Science | 2010-06Hepatitis C virus encodes two enveloped glycoproteins, E1 and E2, which are involved in viral attachment and entry into target cells. We have obtained in insect cells infected by recombinant baculovirus a chimeric secreted recombinant protein, E[...]texto impreso
Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Ortega, Sara ; Lombana, Laura ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Peterson, Darrell L. ; Gavilanes, Francisco | Wiley-Blackwell | 2014-06-01We have used an isolated chimeric protein E1340E2661 that includes the ectodomains of the envelope proteins of epatitis C virus to study its interaction with model membranes. E1340E2661 has some of the membrane destabilization properties, vesicl[...]texto impreso
Tello, Daniel ; Rodríguez-Rodríguez, Mar ; Yélamos, Belén ; Gómez-Gutiérrez, Julián ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2015-03-01In this report it is described for the first time the expression and purification of large quantities of a oluble and correctly folded chimeric recombinant protein, E2661E1340, containing the permuted Hepatitis C virus (HCV) glycoprotein ectodom[...]texto impreso
Rodríguez-Rodríguez, Mar ; Tello, Daniel ; Yélamos, Belén ; GómezGutiérrez, Julián ; Pacheco, Beatriz ; Ortega, Sara ; Serrano, Alicia G. ; Peterson, Darrell L. ; Gavilanes, Francisco | Elsevier Science BV | 2009-01We describe the structural and antigenic properties of a soluble form of hepatitis C virus E2 envelope protein ectodomain ending at residue 661 (E2661) which is obtained in large quantities in a baculovirus/insect cell system. The protein is sec[...]
