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Título:
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Functional organization of the HIV lipid envelope
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Autores:
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Huarte, Nerea ;
Carravilla, Pablo ;
Cruz Rodríguez, Antonio ;
Lorizate, Maier ;
Nieto-Garay, Jon A. ;
Kräusslich, Hans-Georg ;
Pérez Gil, Jesús ;
Requejo Isidro, José ;
Nieva, José L.
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Tipo de documento:
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texto impreso
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Editorial:
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Nature Publishing Group, 2016
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Dimensiones:
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application/pdf
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Nota general:
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cc_by
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Biología: Bioquímica
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Tipo = Artículo
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Resumen:
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The chemical composition of the human immunodeficiency virus type 1 (HIV-1) membrane is critical for fusion and entry into target cells, suggesting that preservation of a functional lipid bilayer organization may be required for efficient infection. HIV-1 acquires its envelope from the host cell plasma membrane at sites enriched in raft-type lipids. Furthermore, infectious particles display aminophospholipids on their surface, indicative of dissipation of the inter-leaflet lipid asymmetry metabolically generated at cellular membranes. By combining two-photon excited Laurdan fluorescence imaging and atomic force microscopy, we have obtained unprecedented insights into the phase state of membranes reconstituted from viral lipids (i.e., extracted from infectious HIV-1 particles), established the role played by the different specimens in the mixtures, and characterized the effects of membrane-active virucidal agents on membrane organization. In determining the molecular basis underlying lipid packing and lateral heterogeneity of the HIV-1 membrane, our results may help develop compounds with antiviral activity acting by perturbing the functional organization of the lipid envelope.
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En línea:
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https://eprints.ucm.es/43222/1/Huarte%2C%20N.%20Functional%20organization%20of%20the%20HIV.pdf
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