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Velasco Bucheli, Rodrigo ; Cerro, Carlos del ; Hormigo, Daniel ; Acebal Sarabia, Carmen ; Arroyo Sánchez, Miguel ; García, José Luis ; Mata Riesco, Isabel de la | Cross Mark | 2015-01-08Here, we describe the draft genome sequence of Actinoplanes utahensis NRRL 12052, a filamentous bacterium that encodes an aculeacin A acylase and a putative N-acyl-homoserine lactone acylase of biotechnological interest. Moreover, several nonrib[...]texto impreso
Hormigo, Daniel ; López Conejo, María Teresa ; Serrano Aguirre, Lara ; García Martín, Alberto ; Saborido Modia, Ana ; Mata Riesco, Isabel de la ; Arroyo Sánchez, Miguel | Taylor & Francis | 2019-08-14Enzymatic synthesis of penicillin V (penV) by acylation of 6-aminopenicillanic acid (6-APA) was carried out using methyl phenoxyacetate (MPOA) as activated acyl donor and soluble penicillin acylase from Streptomyces lavendulae (SlPVA) as biocata[...]texto impreso
Martínez, Virginia ; Gómez de Santos, Patricia ; García Hidalgo, Javier ; Hormigo, Daniel ; Prieto, M. Auxiliadora ; Arroyo Sánchez, Miguel ; Mata Riesco, Isabel de la | Cross Mark | 2015-07-10Cloning and biochemical characterization of a novel extracellular medium-chain-length polyhydroxyalkanoate (mcl-PHA) depolymerase from Streptomyces exfoliatus K10 DSMZ 41693 are described. The primary structure of the depolymerase (PhaZSex2) inc[...]texto impreso
Torres Bacete, Jesús ; Hormigo, Daniel ; Torres Guzmán, Raquel ; Arroyo Sánchez, Miguel ; Castillón, María Pilar ; García, José Luis ; Acebal Sarabia, Carmen ; Mata Riesco, Isabel de la | ASM | 2015-02The pva gene from Streptomyces lavendulae ATCC 13664, encoding a novel penicillin V acylase (SlPVA), has been isolated and characterized. The gene encodes an inactive precursor protein containing a secretion signal peptide that is activated by t[...]texto impreso
Velasco Bucheli, Rodrigo ; Hormigo, Daniel ; Fernández-Lucas, Jesús ; Torres Ayuso, Pedro ; Alfaro Ureña, Yohana ; Saborido Modia, Ana ; Serrano Aguirre, Lara ; García, José Luis ; Ramón, Fernando ; Acebal Sarabia, Carmen ; Santos de la Sen, Antonio ; Arroyo Sánchez, Miguel ; Mata Riesco, Isabel de la | MDPI | 2020-07-01Many Gram-negative bacteria produce N-acyl-homoserine lactones (AHLs), quorum sensing (QS) molecules that can be enzymatically inactivated by quorum quenching (QQ) processes; this approach is considered an emerging antimicrobial alternative. In [...]
