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Título:
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Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.
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Autores:
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Clemente, Luis Felipe ;
Hernaez, Maria Luisa ;
Ramos-Fernández, Antonio ;
Ligero, Gertrudis ;
Gil, Concha ;
Corrales, Fernando José ;
Marcilla, Miguel
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Tipo de documento:
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texto impreso
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Editorial:
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American Chemical Society, 2018-07-05
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Farmacia: Microbiología
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Tipo = Artículo
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Resumen:
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Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.
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En línea:
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https://eprints.ucm.es/55669/1/Clemente%20F%20et%20al%20para%20el%20repositorio.pdf
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