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Título:
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Rapid identification and differentiation of Mycobacterium avium subspecies paratuberculosis types by use of real-time PCR and high-resolution melt analysis of the MAP1506 locus
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Autores:
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Castellanos, Elena ;
Aranaz Martín, Alicia ;
De Buck, Jeroen
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Tipo de documento:
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texto impreso
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Editorial:
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American Society for Microbiology, 2010-04
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/restrictedAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Veterinaria
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Tipo = Artículo
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Resumen:
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High-resolution melt (HRM) analysis can identify sequence polymorphisms by comparing the melting curves of amplicons generated by real-time PCR amplification. We describe the application of this technique to identify Mycobacterium avium subspecies paratuberculosis types I, II, and III. The HRM approach was based on type-specific nucleotide sequences in MAP1506, a member of the PPE (proline-proline-glutamic acid) gene family.
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En línea:
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https://eprints.ucm.es/39691/1/359.pdf
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