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Título:
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Analysis of intermediates of steroid transformations in resting cells by thin-layer chromatography (TLC)
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Autores:
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Guevara, Govinda ;
Perera González, Jesús ;
Navarro Llorens, Juana-María
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Tipo de documento:
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texto impreso
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Editorial:
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Springer, 2017
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/restrictedAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Biología: Bioquímica
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Tipo = Sección de libro
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Resumen:
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Thin-layer chromatography (TLC) is a useful and convenient method for the analysis of steroids due to: its simple sample preparation, low time-consuming process, high sensitivity, low equipment investment and capacity to work on many samples simultaneously. Here we describe a TLC easy protocol very useful to analyze steroid molecules derived from a biotransformation carried out in wild-type and mutant resting cells of Rhodococcus ruber strain Chol-4. Following this protocol, we were able to detect the presence or the absence of some well-known intermediates of cholesterol catabolism in Rhodococcus, namely AD, ADD, and 9OHAD.
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En línea:
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https://eprints.ucm.es/44684/1/Guevara%2C%20G.%20et%20al.%202017.%20Analysis%20of%20intermediates%20of%20steroid.....pdf
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