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Título:
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Ghrelin Facilitates GLUT2-, SGLT1- and SGLT2-mediated Intestinal Glucose Transport in Goldfish (Carassius auratus)
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Autores:
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Blanco Imperiali, Ayelén M. ;
Bertucci, Juan I. ;
Ramesh, Naresh ;
Delgado Saavedra, María Jesús ;
Valenciano González, Ana Isabel ;
Unniappan, Suraj
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Tipo de documento:
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texto impreso
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Editorial:
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Springer Nature, 2017-03-24
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Dimensiones:
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application/pdf
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Nota general:
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cc_by
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Biología
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Materia = Ciencias Biomédicas: Biología: Fisiología animal
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Materia = Ciencias Biomédicas: Biología: Peces
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Tipo = Artículo
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Resumen:
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Glucose homeostasis is an important biological process that involves a variety of regulatory mechanisms. This study aimed to determine whether ghrelin, a multifunctional gut-brain hormone, modulates intestinal glucose transport in goldfish (Carassius auratus). Three intestinal glucose transporters, the facilitative glucose transporter 2 (GLUT2), and the sodium/glucose co-transporters 1 (SGLT1) and 2 (SGLT2), were studied. Immunostaining of intestinal sections found colocalization of ghrelin and GLUT2 and SGLT2 in mucosal cells. Some cells containing GLUT2, SGLT1 and SGLT2 coexpressed the ghrelin/growth hormone secretagogue receptor 1a (GHS-R1a). Intraperitoneal glucose administration led to a significant increase in serum ghrelin levels, as well as an upregulation of intestinal preproghrelin, ghrelin O-acyltransferase and ghs-r1 expression. In vivo and in vitro ghrelin treatment caused a concentration- and time-dependent modulation (mainly stimulatory) of GLUT2, SGLT1 and SGLT2. These effects were abolished by the GHS-R1a antagonist [D-Lys3]-GHRP-6 and the phospholipase C inhibitor U73122, suggesting that ghrelin actions on glucose transporters are mediated by GHS-R1a via the PLC/PKC signaling pathway. Finally, ghrelin stimulated the translocation of GLUT2 into the plasma membrane of goldfish primary intestinal cells. Overall, data reported here indicate an important role for ghrelin in the modulation of glucoregulatory machinery and glucose homeostasis in fish.
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En línea:
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https://eprints.ucm.es/id/eprint/44346/1/Delgado.%202017.%20Ghrelin%20Facilitates%20GLUT2-%2C%20SGLT1-and%20SGLT2-mediated%20Intestinal%20Glucose%20Transport%20in%20Goldfish%20%28Carassius%20auratus%29.pdf
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