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Título:
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1,3-Propanediol production by Klebsiella oxytoca NRRL-B199 from glycerol. Medium composition and operational conditions
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Autores:
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Wojtusik, Mateusz ;
Rodríguez Martín, Alberto ;
Ripoll Morales, Vanessa ;
Santos Mazorra, Victoria E. ;
García, Jose L. ;
García-Ochoa Soria, Félix
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Tipo de documento:
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texto impreso
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Editorial:
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Elsevier, 2015
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Dimensiones:
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application/pdf
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Nota general:
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cc_by_nc_nd
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias: Química: Ingeniería química
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Tipo = Artículo
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Resumen:
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Production of 1,3-propanediol from glycerol using Klebsiella oxytoca NRRL-B199 has been studied. Medium composition has been optimized by means of a statistical design based on the Taguchi method. Strong influences of glycerol and phosphate concentrations have been detected on biomass and product yields. Other factors, such as magnesium concentration and K:Na ratio, have shown a small influence on both responses, biomass and product concentrations. An optimized medium composition has been proposed, leading to a final 1,3-propanediol concentration of 12.4 g/L with a selectivity of 72% with respect to glycerol consumed at shaken bottle-scale. Once the medium composition had been optimized, the scale-up from shaken bottles to STBR was conducted. Several experiments in a 2 L STBR have been conducted in order to determine the best operating conditions concerning temperature and agitation. Under the best operating conditions, i.e., a programmed variable stirring rate ranging from 50 to 100 rpm and a temperature of 37 ?C, a final concentration of 13.5 g/L of 1,3-propanediol with a selectivity of 86% with respect to the glycerol consumed was obtained.
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En línea:
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https://eprints.ucm.es/id/eprint/29624/1/victoria%20santos.pdf
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