|
Título:
|
Effect of lung surfactant Protein SP-C and SP-C-Promoted membrane fragmentation on cholesterol dynamics
|
|
Autores:
|
Roldán López, Nuria ;
Nyholm, Thomas K. M. ;
Slotte, J. Peter ;
Pérez Gil, Jesús ;
García Álvarez, Begoña
|
|
Tipo de documento:
|
texto impreso
|
|
Editorial:
|
BIOPHYSICAL SOCIETY, 2016-10-18
|
|
Dimensiones:
|
application/pdf
|
|
Nota general:
|
info:eu-repo/semantics/restrictedAccess
|
|
Idiomas:
|
|
|
Palabras clave:
|
Estado = Publicado
,
Materia = Ciencias Biomédicas: Biología: Bioquímica
,
Tipo = Artículo
|
|
Resumen:
|
To allow breathing and prevent alveolar collapse, lung surfactant (LS) develops a complex membranous system at the respiratory surface. LS is defined by a specific protein and lipid composition, including saturated and unsaturated phospholipid species and cholesterol. Surfactant protein C (SP-C) has been suggested to be an essential element for sustaining the presence of cholesterol in surfactant without functional impairment. In this work, we used a fluorescent sterol-partitioning assay to assess the effect of the surfactant proteins SP-B and SP-C on cholesterol distribution in membranes. Our results suggest that in the LS context, the combined action of SP-B and SP-C appears to facilitate cholesterol dynamics, whereas SP-C does not seem to establish a direct interaction with cholesterol that could increase the partition of free cholesterol into membranes. Interestingly, SP-C exhibits a membrane-fragmentation behavior, leading to the conversion of large unilamellar vesicles into highly curved vesicles ~25 nm in diameter. Sterol partition was observed to be sensitive to the bending of bilayers, indicating that the effect of SP-C to mobilize cholesterol could be indirectly associated with SP-C-mediated membrane remodeling. Our results suggest a potential role for SP-C in generating small surfactant structures that may participate in cholesterol mobilization and pulmonary surfactant homeostasis at the alveolar interfaces.
|
|
En línea:
|
https://eprints.ucm.es/43462/1/Roldan%2C%20N.Effect%20of%20lung%20surfactant%20protein%20SP-C.pdf
|
