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Título:
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Immobilized enzyme reactors based on nucleoside phosphorylases and 2?-deoxyribosyltransferase for the in-flow synthesis of pharmaceutically relevant nucleoside analogues
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Autores:
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Rinaldi, Francesca ;
Fernández-Lucas, Jesús ;
Fuente, Diego de la ;
Zheng, Changping ;
Bavaro, Teodora ;
Peters, Benjamin ;
Massolini, Gabriella ;
Annunziata, Francesca ;
Conti, Paola ;
Mata, Isabel de la ;
Terreni, Marco ;
Calleri, Enrica
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Tipo de documento:
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texto impreso
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Editorial:
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Elsevier, 2020-03-26
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/restrictedAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Biología: Bioquímica
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Tipo = Artículo
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Resumen:
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In this work, a mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) were developed as prototypes for biosynthetic purposes and their performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest were evaluated. Two biocatalytic routes based on nucleoside 2?-deoxyribosyltransferase from Lactobacillus reuteri (LrNDT) and uridine phosphorylase from Clostridium perfrigens (CpUP)/purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP) were investigated in the synthesis of 2?-deoxy, 2?,3?- dideoxy and arabinonucleoside derivatives. LrNDT-IMER catalyzed the synthesis of 5-fluoro-2?-deoxyuridine and 5-iodo-2?-deoxyuridine in 65–59% conversion yield, while CpUP/AhPNP-IMER provided the best results for the preparation of arabinosyladenine (60% conversion yield).
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En línea:
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https://eprints.ucm.es/id/eprint/62669/1/Rinaldi%2C%20Francesca%20et%20al.%202020.%20Immobilized%20enzyme%20reactors%20based%20on%20nuceoside.....pdf
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