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Título:
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The ribotoxin ?-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes
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Autores:
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Olombrada, Miriam ;
Peña, Cohue ;
Rodríguez Galán, Olga ;
Klingauf Nerurkar, Purnima ;
Portugal Calisto, Daniela ;
Oborská Oplová, Michaela ;
Altvater, Martin ;
Gavilanes, José G. ;
Martínez del Pozo, Álvaro ;
De la Cruz, Jesús ;
García Ortega, Lucía ;
Govind Panse, Vikram
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Tipo de documento:
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texto impreso
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Editorial:
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Elsevier, 2020-04-04
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Dimensiones:
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application/pdf
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Nota general:
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cc_by_nc
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias: Química: Biología molecular
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Materia = Ciencias: Química: Bioquímica
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Tipo = Artículo
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Resumen:
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The ribotoxin -sarcin belongs to a family of ribonucleases that cleave the sarcin/ricin loop (SRL), a critical functional rRNA element within the large ribosomal subunit (60S), thereby abolishing translation. Whether -sarcin targets the SRL only in mature 60S subunits remains unresolved. Here, we show that, in yeast, -sarcin can cleave SRLs within late 60S pre-ribosomes containing mature 25S rRNA but not nucleolar/nuclear 60S pre-ribosomes containing 27S pre-rRNA in vivo. Conditional expression of -sarcin is lethal, but does not impede early pre-rRNA processing, nuclear export and the cytoplasmic maturation of 60S pre-ribosomes. Thus, SRL-cleaved containing late 60S pre-ribosomes seem to escape cytoplasmic proofreading steps. Polysome analyses revealed that SRL-cleaved 60S ribosomal subunits form 80S initiation complexes, but fail to progress to the step of translation elongation. We suggest that the functional integrity of a -sarcin cleaved SRL might be assessed only during translation.
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En línea:
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https://eprints.ucm.es/id/eprint/62188/1/Olombrada2020_NucleicAcidsResearch.pdf
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