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Título:
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Electrochemical sensor for rapid determination of fibroblast growth factor receptor 4 in raw cancer cell lysates
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Autores:
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Khodarahmi, Reza ;
Torrente Rodríguez, Rebeca M. ;
Ruiz-Valdepeñas Montiel, Víctor ;
Campuzano, Susana ;
Pedrero Muñoz, María ;
Farchado, Meryem ;
Vargas, Eva ;
Manuel de Villena, F. Javier ;
Garranzo Asensio, María ;
Barderas, Rodrigo ;
Pingarrón Carrazón, José Manuel
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Tipo de documento:
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texto impreso
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Editorial:
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San Francisco, CA : Public Library of Science, 2017
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Dimensiones:
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application/pdf
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Nota general:
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cc_by
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias: Química: Química analítica
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Tipo = Artículo
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Resumen:
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The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 ?g of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.
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En línea:
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https://eprints.ucm.es/44543/1/Paper%20FGFR4%20PLOS%20ONE%202017.pdf
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