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Título:
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Metabolic engineering of Rhodococcus ruber Chol-4: A cell factory for testosterone production
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Autores:
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Guevara, Govinda ;
Olortegui Flores, Yamileth ;
Fernández de las Heras, Laura ;
Perera González, Julián ;
Navarro LLorens, Juana María
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Tipo de documento:
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texto impreso
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Editorial:
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Public Library of Science, 2019-07-26
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Dimensiones:
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application/pdf
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Nota general:
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cc_by
info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias Biomédicas: Biología: Bioquímica
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Materia = Ciencias Biomédicas: Biología: Microbiología
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Tipo = Artículo
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Resumen:
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Rhodococcus ruber Chol-4 is a potent steroid degrader that has a great potential as a biotechnological tool. As proof of concept, this work presents testosterone production from 4- androstene-3,17-dione by tailoring innate catabolic enzymes of the steroid catabolism inside the strain. A R. ruber quadruple mutant was constructed in order to avoid the breakage of the steroid nucleus. At the same time, an inducible expression vector for this strain was developed. The 17-ketoreductase gene from the fungus Cochliobolus lunatus was cloned and overexpressed in this vector. The engineered strain was able to produce testosterone from 4-androstene-3,17-dione using glucose for cofactor regeneration with a molar conversion of 61%. It is important to note that 91% of the testosterone was secreted outside the cell after 3 days of cell biotransformation. The results support the idea that Rhodococcus ruber Chol-4 can be metabolically engineered and can be used for the production of steroid intermediates.
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En línea:
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https://eprints.ucm.es/57927/1/Guevara%2C%20G.%20et%20al.%202019.%20Metabolic%20engineeringof%20Rhodococcus....pdf
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