| Título: | IOP induces upregulation of GFAP and MHC-II and microglia reactivity in mice retina contralateral to experimental glaucoma |
| Autores: | Gallego Collado, Beatriz Isabel ; Salazar Corral, Juan José ; Hoz Montañana, María Rosa de ; Rojas López, María Blanca ; Ramírez Sebastián, Ana Isabel ; Salinas Navarro, Manuel Ángel ; Ortín Martínez, Arturo ; Valiente Soriano, Francisco Javier ; Avilés Trigueros, Marcelino ; Villegas Pérez, María Paz ; Vidal Sanz, Manuel ; Triviño Casado, Alberto ; Ramirez Sebastian, Jose Manuel |
| Tipo de documento: | texto impreso |
| Editorial: | Biomed Central Ltd, 2012-05-12 |
| Dimensiones: | application/pdf |
| Nota general: |
cc_by info:eu-repo/semantics/openAccess |
| Idiomas: | |
| Palabras clave: | Estado = Publicado , Materia = Ciencias Biomédicas: Medicina: Microbiología médica , Materia = Ciencias Biomédicas: Medicina: Oftalmología , Materia = Ciencias Biomédicas: Óptica y optometría: Óptica oftálmica , Materia = Ciencias Biomédicas: Óptica y optometría: Anatomía ocular , Tipo = Artículo |
| Resumen: |
Background Ocular hypertension is a major risk factor for glaucoma, a neurodegenerative disease characterized by an irreversible decrease in ganglion cells and their axons. Macroglial and microglial cells appear to play an important role in the pathogenic mechanisms of the disease. Here, we study the effects of laser-induced ocular hypertension (OHT) in the macroglia, microglia and retinal ganglion cells (RGCs) of eyes with OHT (OHT-eyes) and contralateral eyes two weeks after lasering. Methods Two groups of adult Swiss mice were used: age-matched control (naïve, n?=?9); and lasered (n?=?9). In the lasered animals, both OHT-eyes and contralateral eyes were analyzed. Retinal whole-mounts were immunostained with antibodies against glial fibrillary acid protein (GFAP), neurofilament of 200kD (NF-200), ionized calcium binding adaptor molecule (Iba-1) and major histocompatibility complex class II molecule (MHC-II). The GFAP-labeled retinal area (GFAP-RA), the intensity of GFAP immunoreaction (GFAP-IR), and the number of astrocytes and NF-200?+?RGCs were quantified. Results In comparison with naïve: i) astrocytes were more robust in contralateral eyes. In OHT-eyes, the astrocyte population was not homogeneous, given that astrocytes displaying only primary processes coexisted with astrocytes in which primary and secondary processes could be recognized, the former having less intense GFAP-IR (P?0.001); ii) GFAP-RA was increased in contralateral (P? Conclusion The use of the contralateral eye as an internal control in experimental induction of unilateral IOP should be reconsidered. The gliotic behavior in contralateral eyes could be related to the immune response. The absence of NF-200+RGCs (sign of RGC degeneration) leads us to postulate that the MHC-II upregulation in contralateral eyes could favor neuroprotection. |
| En línea: | https://eprints.ucm.es/id/eprint/31083/1/1742-2094-9-92.pdf |
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