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Título:
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Mesoporous Silica Nanoparticles Functionalized withHyaluronic Acid. Effect of the Biopolymer Chain Length onCell Internalization
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Autores:
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Nairi, Valentina ;
Magnolia, Silvia ;
Piludu, Marco ;
Nieddu, Mariella ;
Caria, Cristian Antonio ;
Sogos, Valeria ;
Vallet Regí, María ;
Monduzzi, Maura ;
Salis, Andrea
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Tipo de documento:
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texto impreso
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Editorial:
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Elsevier, 2018-02-12
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias: Química: Materiales
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Materia = Ciencias Biomédicas: Farmacia: Química inorgánica
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Tipo = Artículo
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Resumen:
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Mesoporous silica nanoparticles (MSNs) were functionalized with amino groups (MSN-NH2) and then with hyaluronic acid, a biocompatible biopolymer which can be recognized by CD44 receptors in tumor cells, to obtain a targeting drug delivery system. To this purpose, three hyaluronic acid samples differing for the molecular weight, namely HAS (8 -15 kDa), HAM (30-50 kDa) and HAL (90-130 kDa), were used. The MSN-HAS, MSN-HAM, and MSN-HAL materials were characterized through zeta potential and dynamic light scattering measurements at pH=7.4 and T=37°C to simulate physiological conditions. While zeta potential showed an increasing negative value with the increase of the HA chain length, an anomalous value of the hydrodynamic diameter was observed for MSN-HAL, which was smaller than that of MSN-HAS and MSN-HAM samples. The cellular uptake of MSN-HA samples on HeLa cells at 37°C was studied by optical and electron microscopy. HA chain length affected significantly the cellular uptake that occurred at a higher extent for MSN-NH2 and MSN-HAS than for MSN-HAM and MSN-HAL samples. Cellular uptake experiments carried out at 4°C showed that the internalization process was inhibited for MSN-HA samples but not for MSN-NH2. This suggests the occurrence of two different mechanisms of internalization. For MSN-NH2 the uptake is mainly driven by the attractive electrostatic interaction with membrane phospholipids, while MSN-HA internalization involves CD44 receptors
overexpressed in HeLa cells.
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En línea:
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https://eprints.ucm.es/id/eprint/46432/1/COLSUB_9159%20acepted%20manuscript%20%281%29.pdf
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