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Título:
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Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
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Autores:
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Ojeda Fernández, Irene ;
Moreno Guzmán, María ;
González Cortés, Araceli ;
Yáñez Sedeño, Paloma ;
Pingarrón Carrazón, José Manuel
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Tipo de documento:
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texto impreso
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Editorial:
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Wiley-VCH, 2013
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias: Química: Química analítica
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Tipo = Artículo
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Resumen:
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Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1–1000?µg/mL and 0.040?µg/mL (Protein A-MBs), and 0.025–20?µg/mL and 0.018?µg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.
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En línea:
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https://eprints.ucm.es/44742/1/2013%20Galley%20Proofs%20MBs%20CP.pdf
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