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Título:
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Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes
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Autores:
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Rodrigo Martín-Romo, José Augusto ;
Soto Rueda, Juan Manuel ;
Alieva, Tatiana Krasheninnikova
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Tipo de documento:
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texto impreso
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Editorial:
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Society of Photo-Optical Instrumentation Engineers (SPIE), 2019
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias: Física: Optica
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Tipo = Sección de libro
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Resumen:
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Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limitedangle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a non-homogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed.
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En línea:
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https://eprints.ucm.es/58614/1/AlievaT120libre.pdf
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