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Título:
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Effects of a mesoporous bioactive glass on osteoblasts, osteoclasts and macrophages
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Autores:
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Gómez-Cerezo, Natividad ;
Casarrubios, L. ;
Morales, I. ;
Feito Castellano, María José ;
Vallet Regí, María ;
Arcos Navarrete, Daniel ;
Portolés Pérez, María Teresa
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Tipo de documento:
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texto impreso
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Editorial:
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Elsevier, 2018-10-15
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Dimensiones:
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application/pdf
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Nota general:
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info:eu-repo/semantics/openAccess
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Idiomas:
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Palabras clave:
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Estado = Publicado
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Materia = Ciencias: Química: Materiales
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Materia = Ciencias Biomédicas: Farmacia: Química inorgánica
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Tipo = Artículo
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Resumen:
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A mesoporous bioactive glass (MBG) of molar composition 75SiO2-20CaO-5P2O5 (MBG-75S) has been synthetized as a potential bioceramic for bone regeneration purposes. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption studies and transmission electron microscopy (TEM) demonstrated that MBG-75S possess a highly ordered mesoporous structure with high surface area and porosity, which would explain the high ionic exchange rate (mainly calcium and silicon soluble species) with the surrounded media. MBG-75S showed high biocompatibility in contact with Saos-2 osteoblast-like cells. Concentrations up to 1 mg/ml did not lead to significant alterations on either morphology or cell cycle. Regarding the effects on osteoclasts, MBG-75S allowed the differentiation of RAW264.7 macrophages into osteoclast-like cells but exhibiting a decreased resorptive activity. These results point out that MBG-75S does not inhibit osteoclastogenesis but reduces the osteoclast bone-resorbing capability. Finally, in vitro studies focused on the innate immune response, evidenced that MBG-75S allows the proliferation of macrophages without inducing their polarization towards the M1 pro-inflammatory phenotype. This in vitro behavior is indicative that MBG-75S would just induce the required innate immune response without further inflammatory complications under in vivo conditions. The overall behavior respect to osteoblasts, osteoclasts and macrophages, makes this MBG a very interesting candidate for bone grafting applications in osteoporotic patients.
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En línea:
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https://eprints.ucm.es/id/eprint/47796/1/pagination_YJCIS_23677.pdf
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