|
Título:
|
Parathyroid hormone-related protein (107-111) improves the bone regeneration potential of gelatin-glutaraldehyde biopolymer-coated hydroxyapatite
|
|
Autores:
|
Lozano Borregón, Daniel ;
Sánchez Salcedo, Sandra ;
Portal Núñez, Sergio ;
Vila, Mercedes ;
López Herradón, Ana ;
Ardura, Juan A. ;
Mulero, Francisca ;
Gómez Barrena, Enrique ;
Vallet Regí, María ;
Esbrit, Pedro
|
|
Tipo de documento:
|
texto impreso
|
|
Editorial:
|
Elsevier, 2014-07
|
|
Dimensiones:
|
application/pdf
|
|
Nota general:
|
info:eu-repo/semantics/openAccess
|
|
Idiomas:
|
|
|
Palabras clave:
|
Estado = Publicado
,
Materia = Ciencias: Química: Materiales
,
Materia = Ciencias: Química: Química inorgánica
,
Tipo = Artículo
|
|
Resumen:
|
Biopolymer-coated nanocrystalline hydroxyapatite (HA) made as macroporous foams which are degradable and flexible are promising candidates as orthopaedic implants. The C-terminal (107-111) epitope of parathyroid hormone-related protein (PTHrP) exhibits osteogenic properties. The main aim of this study was to evaluate whether PTHrP (107-111) loading into gelatin-glutaraldehyde biopolymer-coated HA (HA(GIu)) scaffolds would produce an optimal biomaterial for tissue engineering applications. HA(GIu) scaffolds with and without PTHrP (107-111) were implanted into a cavitary defect performed in both distal tibial metaphysis of adult rats. Animals were sacrificed after 4 weeks for histological, microcomputerized tomography and gene expression analysis of the callus. At this time, bone healing occurred only in the presence of PTHrP (107-111)-containing HA(Giu) implant, related to an increase in bone volume/tissue volume and trabecular thickness, cortical thickness and gene expression of osteocalcin and vascular cell adhesion molecule I, but a decreased gene expression of Wnt inhibitors, SOST and dickkopf homolog 1. The autonomous osteogenic effect of the PTHrP (107-111)-loaded HA(Giu) scaffolds was confirmed in mouse and human osteoblastic cell cultures. Our findings demonstrate the advantage of loading PTHrP (107-111) into degradable HA(GIu) scaffolds for achieving an optimal biomaterial that is promising for low load bearing clinical applications. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
|
|
En línea:
|
https://eprints.ucm.es/41105/1/%28618%29%20Acta%20Biomaterialia.%2010%2C%203307%E2%80%933316%20%20%202014%20LOZANO.pdf
|
