| Título: | Dual Roles of Diadenosine Polyphosphates in Corneal Epithelial Cell Migration |
| Autores: | Mediero Muñoz, Aránzazu ; Peral Cerdá, Assumpta ; Pintor, Jesús |
| Tipo de documento: | texto impreso |
| Editorial: | The Association for Research in Vision and Ophthalmology, Inc., 2006-10 |
| Dimensiones: | application/pdf |
| Nota general: |
cc_by_nc_nd info:eu-repo/semantics/openAccess |
| Idiomas: | |
| Palabras clave: | Estado = Publicado , Materia = Ciencias: Química: Química orgánica , Materia = Ciencias Biomédicas: Medicina: Bioquímica , Materia = Ciencias Biomédicas: Medicina: Oftalmología , Tipo = Artículo |
| Resumen: |
Purpose. To investigate the influence of diadenosine polyphosphates on the rate of corneal epithelial cell migration. Methods. Primary corneal epithelial cell cultures were obtained from New Zealand White rabbits. Immunocytochemical experiments were performed by fixing the cells with 4% paraformaldehyde (PFA) and incubated with cytokeratin 3 primary antibody, which was subsequently incubated with a secondary IgG mouse labeled with FITC, and the cells were observed under confocal microscopy. Migration studies were performed by taking confluent monolayers that were wounded with a pipette tip and challenged with different di- and mononucleotides with or without P2 antagonist (n = 8 each treatment). For concentration–response analysis, compounds were tested in doses ranging from 10?8 to 10?3 M (n = 8). The stability of the dinucleotides was assayed by HPLC, with an isocratic method (n = 4). Results. Cells under study were verified as corneal epithelial cells via the immunocytochemical analysis. Cell migration experiments showed that Ap4A, UTP, and ATP accelerated the rate of healing (5, 2.75, and 3 hours, respectively; P Conclusions. Based on the pharmacological profile of all the compounds tested, the two main P2Y receptors that exist in these corneal cells are a P2Y2 receptor accelerating the rate of healing and a P2Y6 receptor that delays this process. |
| En línea: | https://eprints.ucm.es/39547/1/Dual%20role%20diadenosine_IOVS-2006.pdf |
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