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Título:
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HbIDI, SlIDI and EcIDI: A comparative study of isopentenyl diphosphate isomerase activity and structure
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Autores:
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Berthelot, Karine ;
Estevez, Yannick ;
Quiliano, Miguel ;
Baldera-Aguayo, Pedro-A. ;
Zimic, Mirko ;
Pribat, Anne ;
Bakleh, Marc-Elias ;
Teyssier, Emeline ;
Gallusci, Philippe ;
Gardrat, Christian ;
Lecomte, Sophie ;
Peruch, Frederic
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Tipo de documento:
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texto impreso
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Editorial:
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Elsevier, 2019-02-06T14:45:11Z
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Nota general:
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info:eu-repo/semantics/restrictedAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
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Idiomas:
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Inglés
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Palabras clave:
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Editados por otras instituciones
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Artículos
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Artículos en revistas indizadas
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Resumen:
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In this study, we cloned, expressed and purified the isopentenyl diphosphate isomerases (IDIs) from two plants, Hevea brasiliensis and Solanum lycopersicum, and compared them to the already well characterized Escherichia coli IDI. Phylogenetic analysis showed high homology between the three enzymes. Their catalytic activity was investigated in vitro with recombinant purified enzymes and in vivo by complementation colorimetric tests. The three enzymes displayed consistent activities both in vitro and in vivo. In term of structure, studied by
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En línea:
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http://doi.org/10.1016/j.biochi.2016.05.005
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