Título:
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In Vitro Cytotoxicity Evaluation of Three Root-End Filling Materials in Human Periodontal Ligament Fibroblasts
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Autores:
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Coaguila-Llerena, Hernan ;
Vaisberg, Abraham ;
Velasquez-Huaman, Zulema
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Tipo de documento:
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texto impreso
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Editorial:
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Fundação Odontológica de Ribeirão Preto, 2019-02-06T14:45:12Z
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Nota general:
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info:eu-repo/semantics/restrictedAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
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Idiomas:
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Inglés
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Palabras clave:
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Editados por otras instituciones
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Resumen:
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The aim of this study was to evaluate in vitro the cytotoxicity on human periodontal ligament fibroblasts of three root-end filling materials: MTA Angelus(R), EndoSequence Root Repair Material Putty(R) and Super EBA(R). A primary culture of human periodontal ligament fibroblasts was previously obtained in order to evaluate the cytotoxicity of the three extracts from the root-end filling materials after 2 and 7 days of setting. Serial dilutions of these extracts (1:1, 1:2, 1:4 and 1:8) were evaluated at 1, 3 and 7 days using the methyl-thiazol-tetrazolium (MTT) colorimetric assay. Cell viability was evaluated as percentage of the negative control group, which represented 100% cell viability. Statistical analyses were done with t-test, ANOVA and Kruskal-Wallis test at a significance level of 5%. It was found that the main difference among root-end filling materials was in the higher dilutions (p0.05). Cell viability of MTA Angelus(R) was superior for 2-day setting (p
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En línea:
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http://doi.org/10.1590/0103-6440201600447
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