|
Título:
|
Predominant Mycobacterium tuberculosis families and high rates of recent transmission among new cases are not associated with primary multidrug resistance in Lima, Peru
|
|
Autores:
|
Barletta, F. ;
Otero, L. ;
de Jong, B.C. ;
Iwamoto, T. ;
Arikawa, K. ;
Van der Stuyft, P. ;
Niemann, S. ;
Merker, M. ;
Uwizeye, C. ;
Seas, C. ;
Rigouts, L.
|
|
Tipo de documento:
|
texto impreso
|
|
Editorial:
|
American Society for Microbiology, 2019-04-24T18:23:53Z
|
|
Nota general:
|
info:eu-repo/semantics/restrictedAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
|
|
Idiomas:
|
Inglés
|
|
Palabras clave:
|
Editados por otras instituciones
,
Artículos
,
Artículos en revistas indizadas
|
|
Resumen:
|
Sputum samples from new tuberculosis (TB) cases were collected over 2 years as part of a prospective study in the northeastern part of Lima, Peru. To measure the contribution of recent transmission to the high rates of multidrug resistance (MDR) in this area, Mycobacterium tuberculosis complex (MTBc) isolates were tested for drug susceptibility to first-line drugs and were genotyped by spoligotyping and 15-locus mycobacterial interspersed repetitive-unit (MIRU-15)-variable-number tandem repeat (VNTR) analysis. MDR was found in 6.8% of 844 isolates, of which 593 (70.3%) were identified as belonging to a known MTBc lineage, whereas 198 isolates (23.5%) could not be assigned to these lineages and 12 (1.4%) represented mixed infections. Lineage 4 accounted for 54.9% (n=463) of the isolates, most of which belonged to the Haarlem family (n=279). MIRU-15 analysis grouped 551/791 isolates (69.7%) in 102 clusters, with sizes ranging from 2 to 46 strains. The overall high clustering rate suggests a high level of recent transmission in this population, especially among younger patients (odds ratio [OR], 1.6; P=0.01). Haarlem strains were more prone to cluster, compared to the other families taken together (OR, 2.0; P
|
|
En línea:
|
http://doi.org/10.1128/JCM.03585-14
|
